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KMID : 0894520110150020113
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Development & Reproduction
2011 Volume.15 No. 2 p.113 ~ p.120
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BCL2L10 Protein Induces Apoptosis in KGN-Human Granulosa Cells
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Kim Jae-Hong
Lee Kyung-Ah
Bae Jee-Hyeon
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Abstract
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BCL-2 family essential proteins to play a pivotal role to perform in apoptosis signaling pathways and essential proteins for the regulation of cell death. BCL2L10 protein is a member of BCL-2 family and it regulates both anti-apoptotic and pro-apoptotic function of specific tissue or cell line. BCL2L10 of function and expression is not reported in ovary cell lines. In this study we reported that BCL2L10 were significant expression of KGN cell line. Ectopic expression of BCL2L10 induced cell death, and its cells killing effect was blocked by pan-caspase inhibitor of the Z-VAD-fmk. Ectopic expression of BCL2L10 protein led to the activation of caspase 9 and caspase 3, suggesting apoptotic cell death, and confocal microscopic analyses showed that BCL2L10 was partially localized in mitochondria. Thus, we provide a novel function of BCL2L10 in KGN cells, which was involved in the intrinsic cell death pathway.
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KEYWORD
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Apoptosis, BCL2 family, BCL2L10, Granulosa tumor cell (KGN)
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